Efficient Test Set Modification for Capture Power Reduction

The occurrence of high switching activity when the response to a test vector is captured by flipflops in scan testing may cause excessive IR drop, resulting in significant test-induced yield loss. This paper addresses the problem with a novel method based on test set modification, featuring (1) a new constrained X-identification technique that turns a properly selected set of bits in a fullyspecified test set into X-bits without fault coverage loss, and (2) a new LCP (low capture power) X-filling technique that optimally assigns 0’s and 1’s to the X-bits for the purpose of reducing the switching activity of the resulting test set in capture mode. This method can be readily applied in any test generation flow for capture power reduction without any impact on area, timing, test set size, and fault coverage

Oral History Interview with Yoshihiro Minamoto, September 15, 2005

Transcript of an oral interview with Major General Yoshihiro Minamoto. Through the work of the translator, Minamoto recalls attending a military academy in Tokyo and granduating in April, 1944. From there, he was assigned to a Japanese Army shipping division in Hiroshima. Through the translator, Minamoto describes training a special squadron of suicide boat operators for deployment on Okinawa. He arrived on Okinawa in September, 1944. Minamoto never got a chance to attack with his suicide boat squadron at Okinawa, so he was folded into an infantry unit already on Okinawa. Minamoto also describes surrendering once he heard the war was finished. When he returned to Japan after the war, he volunteered for service in the Japanese Defense Forces

Efficient Test Set Modification for Capture Power Reduction

The occurrence of high switching activity when the response to a test vector is captured by flipflops in scan testing may cause excessive IR drop, resulting in significant test-induced yield loss. This paper addresses the problem with a novel method based on test set modification, featuring (1) a new constrained X-identification technique that turns a properly selected set of bits in a fullyspecified test set into X-bits without fault coverage loss, and (2) a new LCP (low capture power) X-filling technique that optimally assigns 0’s and 1’s to the X-bits for the purpose of reducing the switching activity of the resulting test set in capture mode. This method can be readily applied in any test generation flow for capture power reduction without any impact on area, timing, test set size, and fault coverage

High-Speed Atomic Force Microscopy Reveals Loss of Nuclear Pore Resilience as a Dying Code in Colorectal Cancer Cells

Nuclear pore complexes (NPCs) are the sole turnstile implanted in the nuclear envelope (NE), acting as a central nanoregulator of transport between the cytosol and the nucleus. NPCs consist of ∼30 proteins, termed nucleoporins. About one-third of nucleoporins harbor natively unstructured, intrinsically disordered phenylalanine-glycine strings (FG-Nups), which engage in transport selectivity. Because the barriers insert deeply in the NPC, they are nearly inaccessible. Several <i>in vitro</i> barrier models have been proposed; however, the dynamic FG-Nups protein molecules themselves are imperceptible <i>in vivo</i>. We show here that high-speed atomic force microscopy (HS-AFM) can be used to directly visualize nanotopographical changes of the nuclear pore inner channel in colorectal cancer (CRC) cells. Furthermore, using MLN8237/alisertib, an apoptotic and autophagic inducer currently being tested in relapsed cancer clinical trials, we unveiled the functional loss of nucleoporins, particularly the deformation of the FG-Nups barrier, in dying cancer cells. We propose that the loss of this nanoscopic resilience is an irreversible dying code in cells. These findings not only illuminate the potential application of HS-AFM as an intracellular nanoendoscopy but also might aid in the design of future nuclear targeted nanodrug delivery tailored to the individual patient

High-Speed Atomic Force Microscopy Reveals Loss of Nuclear Pore Resilience as a Dying Code in Colorectal Cancer Cells

Nuclear pore complexes (NPCs) are the sole turnstile implanted in the nuclear envelope (NE), acting as a central nanoregulator of transport between the cytosol and the nucleus. NPCs consist of ∼30 proteins, termed nucleoporins. About one-third of nucleoporins harbor natively unstructured, intrinsically disordered phenylalanine-glycine strings (FG-Nups), which engage in transport selectivity. Because the barriers insert deeply in the NPC, they are nearly inaccessible. Several <i>in vitro</i> barrier models have been proposed; however, the dynamic FG-Nups protein molecules themselves are imperceptible <i>in vivo</i>. We show here that high-speed atomic force microscopy (HS-AFM) can be used to directly visualize nanotopographical changes of the nuclear pore inner channel in colorectal cancer (CRC) cells. Furthermore, using MLN8237/alisertib, an apoptotic and autophagic inducer currently being tested in relapsed cancer clinical trials, we unveiled the functional loss of nucleoporins, particularly the deformation of the FG-Nups barrier, in dying cancer cells. We propose that the loss of this nanoscopic resilience is an irreversible dying code in cells. These findings not only illuminate the potential application of HS-AFM as an intracellular nanoendoscopy but also might aid in the design of future nuclear targeted nanodrug delivery tailored to the individual patient

Hokuriku-plus familial hypercholesterolaemia registry study: rationale and study design

Introduction Familial hypercholesterolaemia (FH) is an autosomal-dominant inherited genetic disease. It carries an extremely high cardiovascular risk associated with significantly elevated low-density lipoprotein (LDL) cholesterol. The diagnostic rate of this disease in some European nations is quite high, due to the presence of multiple prospective registries. On the other hand, few data—and in particular multicentre data—exist regarding this issue among Japanese subjects. Therefore, this study intends to assemble a multicentre registry that aims to comprehensively assess cardiovascular risk among Japanese FH patients while taking into account their genetic backgrounds.Methods and analysis The Hokuriku-plus FH registry is a prospective, observational, multicentre cohort study, enrolling consecutive FH patients who fulfil the clinical criteria of FH in Japan from 37 participating hospitals mostly in Hokuriku region of Japan from April 2020 to March 2024. A total of 1000 patients will be enrolled into the study, and we plan to follow-up participants over 5 years. We will collect clinical parameters, including lipids, physical findings, genetic backgrounds and clinical events covering atherosclerotic and other important events, such as malignancies. The primary endpoint of this study is new atherosclerotic cardiovascular disease (ASCVD) events. The secondary endpoints are as follows: LDL cholesterol, secondary ASCVD events and the occurrence of other diseases including hypertension, diabetes and malignancies.Ethics and dissemination This study is being conducted in compliance with the Declaration of Helsinki, the Ethical Guidelines for Medical and Health Research Involving Human Subjects, and all other applicable laws and guidelines in Japan. This study protocol has been approved by the Institutional Review Board at Kanazawa University. We will disseminate the final results at international conferences and in a peer-reviewed journal.Trial registration number UMIN000038210